Construction of Two Suppression Subtractive Hybridization Libraries and Identification of Salt-Induced Genes in Soybean

Construction of suppression subtractive hybridization libraries and identification of brown planthopper-induced genes.

A suppression subtractive hybridization technique was used to screen for brown planthopper (BPH)-inducible genes in rice (Oryza sativa). cDNAs from a BPH-resistant rice line (B5) infested by BPH were used as the tester population, and mixed cDNAs from a BPH-sensitive line (MH63) and a control (uninfested B5) as the driver population. After hybridizing and cloning, forward and reverse subtractio...

Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean.

The development of a genetic transformation system is needed to address the problem of the low efficiency associated with soybean regeneration. To contribute to the enhancement of the soybean regenerative capacity, we explored the developmental mechanisms of soybean regeneration at the molecular level using a suppression subtractive hybridization cDNA library constructed from cotyledonary nodes...

Subtractive hybridization and construction of cDNA libraries.

A secondary suppression subtractive hybridization method for isolation and identification of some salt-induced genes in soybean (Glycine max L. Merr)

The yield of soybean (Glycine max L. Merr.) has been seriously challenged by increased salinization of the land. Continuous efforts are needed to understand the salt tolerance mechanism in soybean using suitable biotechnological tools. Although the suppression subtractive hybridization (SSH) method was a powerful technique and has been successful in some cases, it may retain some less effective...

Identification of early salt stress response genes in tomato root by suppression subtractive hybridization and microarray analysis.

High salinity is one of the most serious threats to crop production. To understand the molecular basis of plant responses to salt stress better, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potential important or novel genes involved in the early stage of tomato responses to severe salt stress. First, SSH libraries were constructed for the ...